ANNEXURE II
Bio Safety Guidelines

1. Entry into Laboratory/work area should be restricted.
2. Staff should be provided with aprons for working in the laboratory.
3. Work surfaces should be disinfected when procedures are completed and at the end of each working day, 0.1% Hypochlorite solution is effective for the same.
4. Gloves should be worn for all manipulations of infectious material: Examination gloves of vinyl or latex must be used in laboratory, ward, operation theatre. General purpose utility gloves (i.e. rubber gloves or household gloves, reusable) must be used while cleaning instruments, decontamination procedures and other activities where manual dexterity is not required.
5. In operation theatres and delivery rooms, cleaning must be carried out every day.
6. Cleaning with carbolic acid/phenol has to be carried out every week and swabs should be sent to laboratory for cultures. Fumigation must be done in case cultures turn out positive. Records for the same should be maintained so that they can be scrutinized periodically. All horizontal surfaces including floor should be mopped between cases.
7. All medical instruments should be soaked for 30 minutes in chemical disinfectant before cleaning. This will give further protection to the personnel from exposure to HIV during the process of cleaning.
8. The best form of disinfection is autoclaving. After this comes boiling for 20 minutes.
9. In practical and field settings, high-level disinfection with chemicals is far less reliable than boiling.
10. Hepatitis B vaccine should be provided for all personnel (staff).